A revisit of high collision energy effects on collision-induced dissociation spectra using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-LIFT-TOF/TOF): application to the sequencing of RNA/DNA chimeras.
Identifieur interne : 000113 ( France/Analysis ); précédent : 000112; suivant : 000114A revisit of high collision energy effects on collision-induced dissociation spectra using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-LIFT-TOF/TOF): application to the sequencing of RNA/DNA chimeras.
Auteurs : Florence Mauger [France] ; Jean-Claude Tabet ; Ivo G. GutSource :
- Rapid communications in mass spectrometry : RCM [ 1097-0231 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, Ions, RNA.
- methods : Sequence Analysis, DNA, Sequence Analysis, RNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
Abstract
High-energy collision-induced dissociation (CID) spectra of isomeric RNA/DNA chimeras using matrix-assisted laser desorption/ionization time-of-flight LIFT mass spectrometry (MALDI-LIFT-TOF/TOF) can potentially be applied for an exhaustive fragment characterization in a nucleic acid sequencing scheme. These chimeras contain deoxynucleotides and at the 3'-end a ribonucleotide with a 3'-phosphate group.
DOI: 10.1002/rcm.6913
PubMed: 24861592
Affiliations:
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pubmed:24861592Le document en format XML
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Gut</name></author></analytic><series><title level="j">Rapid communications in mass spectrometry : RCM</title><idno type="eISSN">1097-0231</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA (chemistry)</term><term>Ions (chemistry)</term><term>RNA (chemistry)</term><term>Sequence Analysis, DNA (methods)</term><term>Sequence Analysis, RNA (methods)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ()</term><term>ARN ()</term><term>Analyse de séquence d'ADN ()</term><term>Analyse de séquence d'ARN ()</term><term>Ions ()</term><term>Spectrométrie de masse MALDI ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA</term><term>Ions</term><term>RNA</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Sequence Analysis, DNA</term><term>Sequence Analysis, RNA</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN</term><term>ARN</term><term>Analyse de séquence d'ADN</term><term>Analyse de séquence d'ARN</term><term>Ions</term><term>Spectrométrie de masse MALDI</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">High-energy collision-induced dissociation (CID) spectra of isomeric RNA/DNA chimeras using matrix-assisted laser desorption/ionization time-of-flight LIFT mass spectrometry (MALDI-LIFT-TOF/TOF) can potentially be applied for an exhaustive fragment characterization in a nucleic acid sequencing scheme. 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